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. Author manuscript; available in PMC: 2011 May 25.
Published in final edited form as: Biochemistry. 2010 May 25;49(20):4349–4360. doi: 10.1021/bi100215b

Figure 4.

Figure 4

Examination of G-actin binding by GSNL-1 variants by non-denaturing polyacrylamide gel electrophoresis. 10 μM G-actin and varying concentrations (0 – 10 μM) of full-length GSNL-1 (A and B), G1G2G3 (C and D), G1G2 (E and F), G1b (G and H), G1a (I and J), or G3G4 (K and L) were incubated alone or in mixtures in the presence of 0.1 mM CaCl2 (left column) or 0.5 mM EGTA (right column) for 30 min, and the samples were analyzed by non-denaturing polyacrylamide gel electrophoresis. Positions of free G-actin are indicated by arrows.