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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Reprod Immunol. 2009 Dec 3;84(1):32–40. doi: 10.1016/j.jri.2009.09.006

Figure 4.

Figure 4

Immuno precipitation of biotinylated human sperm proteins with rabbit antiserum against the HSP70-like membrane protein of Chlamydia trachomatis (ctHSP70).

Biotin-labelled sperm proteins precipitated with preimmune rabbit serum (left) and antiserum (right) against a putative peptide interaction domain of sea urchin sperm receptor (represented by aa 351–368 of ctHSP70). Immuno precipitated surface biotinylated proteins were detected by affinity staining with HRP-avidin. While abundant ~60 kDa and ~80–90 kDa sperm surface antigens were specifically purified with the anti-ctHSP70 antiserum, a common 65 kDa surface antigen was immune-precipitated with both the preimmune serum and the antiserum. However, computer densitometry analysis revealed that approximately four times more biotin labelled 65 kDa protein was isolated with the anti-ctHSP70 antiserum, compared to that precipitated with similar amounts of preimmune serum.