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. 2010 Jun 1;120(7):2537–2548. doi: 10.1172/JCI41277

Figure 7. Addition of excess holo-transferrin restores p21 protein levels in ICN1-transduced Hrb–/– T cell precursors.

Figure 7

(A) Western blot of lysates prepared from Hrb+/+ and Hrb–/– MigR1– and ICN1–transduced thymocytes grown on OP9DL1 cocultures and harvested 5 days after transduction. Lysates blotted for p21 and actin (loading control). (B) Quantitative RT-PCR of mRNA isolated from the Hrb+/+ and Hrb–/– MigR1– and ICN1–transduced thymocytes grown as in panel A. (C) Western blot of lysates from Figure 6G blotted for p21 and actin (loading control). (D) GFP-positive cell number of ICN1- and ICN1+p21–transduced WT hematopoietic precursors cocultured on OP9 stromal beds for 10 days. Data are shown as mean ± SD. ***P < 0.001.