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. 2010 Jun 14;120(7):2457–2473. doi: 10.1172/JCI42285

Figure 5. Low bone mass and/or defective calvarial mineralization in Mkk3–/–Mkk6+/–, p38 inhibitor–treated, p38α-deleted, or p38b–/– mice.

Figure 5

(A, C, D, and E) Skulls from Mkk3–/–Mkk6+/– (A), p38 inhibitor–treated (C), p38α-deleted (D), or p38b–/– mice (E) were analyzed by μCT. The p38 inhibitor (SB203580) and mice with lentiviral cre-induced p38α deletion were treated with the inhibitor or infected starting at P2 and analyzed on P9. Other mice were analyzed at P4. cre recombinase expression in calvaria of p38α pups was analyzed by immunoblotting with anti-EGFP antibody. Images are representative of more than 8 mice per genotype or treatment. Images from females are shown. (B, F) Femurs from female Mkk3–/–Mkk6+/– and p38b–/– mice were analyzed by μCT. Displayed are 3D reconstructions of trabecular bone (upper left), midshaft cortical bone (lower left), and BV/TV, trabecular number, trabecular thickness, and cortical thickness parameters. Values are mean + SD. *Significant difference by Student’s t test, P < 0.05; **P < 0.0005.