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. 2010 Jun 23;120(7):2627–2634. doi: 10.1172/JCI42304

Figure 5. Effects of clinically relevant immunosuppressive therapy 7 days after transplantation.

Figure 5

(A) MPO protein tissue level assessed by Western blotting and (B) biochemical determination of MPO tissue activity in isografts, allografts, and allografts of mice treated with prednisolone, azathioprine, and cyclosporine (Rx). (A) Data are shown as mean ± SEM; n = 3 per group. *P < 0.01 (C) Flow cytometric profiling of leukocytes in the same groups as above. (D and E) Quantitative immunoreactive staining for MPO, Ly-6C, macrophage (F4/80), and neutrophil marker (NIMP-R14) in the 3 groups. n = 3 mice per group, *P < 0.01. Scale bar: 10 μm. (F) Representative MPO-Gd MRI before and 120 minutes after injection. Circles indicate graft. Next to the graft, the bladder showed enhancement, indicating renal excretion of MPO-Gd. No enhancement was seen in the allograft treated with immunosuppressive therapy. (G) Change of CNR before injection versus 2 hours after injection in iso- and allografts of mice with and without treatment. n = 6 per group, *P < 0.05.