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. 2010 Jun 1;120(7):2497–2507. doi: 10.1172/JCI42382

Figure 4. Activated Notch signaling in t(14;15)-negative lymphoma cells.

Figure 4

(A) RT-PCR analysis of pre-Tα (pTα) expression in RNA isolated from the indicated cells. (B) Quantitative RT-PCR for Hes-1 and c-myc in WT thymocytes or indicated primary lymphoma cells normalized to Gapdh. (C) Flow cytometric analysis of CD25 expression in cell lines derived from the indicated primary lymphomas. (D) Growth suppression by GSI. The tumor cell lines derived from the indicated primary lymphoma cells were cultured with or without 1 μM GSI in a 24-well plate for 72 hours. Graph shows the number of trypan blue–negative cells per well. (E) Impaired proliferation, cell cycle arrest, and apoptosis in t(14;15)-negative tumor cell lines treated with GSI. (F) Western blot analysis of the activated form of Notch1 protein in the indicated cell lines. All experiments described in C–F are representative of 6 or more different cell lines derived from at least 2 individual primary lymphomas.