Table 2.
A summary of the antibodies selected in this study.
| Target | Antigen | Antibody name | Clone | VL | VH | Formalin sensitive? | |
|---|---|---|---|---|---|---|---|
| Fusion 1 | 1.1 | Jamc.1 | 1.1.1 | V14-111+J2 | ND | ND | |
| 1.2 | Unc5b | SI1-Unc5b | 1.2.1 | V17-127+J4 | V2-2+D1+J4 | Y | |
| SI2-Unc5b | 1.2.2 | V5-48+J5 | V1-14+D1+J4 | N | |||
| 1.2.3 | Cloning halted | ||||||
| 1.3 | Lrrc4a | 1.3.1 | Clone lost | ||||
| 1.4 | Flrt3 | SI3-Flrt3 | 1.4.1 | V3-7+J1 | V1-66+D2+J3 | N | |
| 1.4.2 | Cloning halted | ||||||
| 1.5 | Appa | No positive clone | |||||
| Fusion 2 | 2.1 | Jamb.1 | No positive clone | ||||
| 2.2 | Jamc.1 | No positive clone | |||||
| 2.3 | Jamc.2 | SI4-Jamc.2 | 2.3.1 | V10-96+J1 | V3-2+D2+J1 | N | |
| 2.4 | Vasn | 2.4.1 | N.D. | V5-6+D2+J2 | ND | ||
| 2.5 | Lrrn1 | SI5-Lrrn1 | 2.5.1 | V4-57+J5 | V1-77+D3+J2 | N | |
The pooled antigens used in the immunizations are numbered, and details of the V, D and J segments identified are listed for each antibody. Antibodies were numerically named with the SI prefix for Sanger Institute.
ND = not determined