Figure 4.

Glucose and xylose released (g/kg dry weight biomass (DWB)) during hydrolysis of untreated (open bars) and ammonia fiber expansion (AFEX)-treated (striped bars) corn silage (0X-CS)-based substrates. AFEX pretreatment was performed at different (a) ammonia to biomass loadings (w/w; DWB) at 90°C, 60% (DWB) moisture content, (b) moisture loadings at 90°C and 1:1 (w/w) ammonia to biomass loading and (c) temperatures at 60% (DWB) moisture loading and 1:1 (w/w) ammonia to biomass loading. Enzymatic hydrolysis was carried out at 50°C, 150 rpm in 15 mL reaction volume using Accellerase (3.1 mg/ g of cellulose) and Stargen (3.8 mg/g of starch). The lines above all bars indicate the maximum possible total glucose and xylose yield based on theoretical glucan and xylan composition.