Table 1.
Quantitative real time PCR and corresponding microarray data of selected genes.
| BME Loci |
Gene Function |
Condition (growth phase) |
Change (Fold) |
|
|---|---|---|---|---|
| qRT-PCR | Microarray | |||
| I 0984 | ABC-Type β-(1,2) Glucan Transporter | ΔvjbR/wt (ES) | -2.5 | -2.1 |
| II 0151 | Flagellar M-Ring Protein, FliF | ΔvjbR/wt (ES) | -7.9 | -2.2 |
| II 1069 | Adhesin, AidA | ΔvjbR/wt (SP) | -1.9 | -1.5 |
| I 0561 | Membrane-Bound Lytic Murein Transglycosylase B | ΔvjbR/wt (SP) | -1.7 | -2.0 |
| II 0025 | Attachment Mediating Protein VirB1 | ΔvjbR/wt (SP) | -4.1 | -2.6 |
| I 0831 | UDP-3-O-[3-hydroxymyristoyl] Glucosamine N-Acyltransferase | wt + AHL/wt (ES) | 2.2 | 2.3 |
| II 0151 | Flagellar M-Ring Protein, FliF | wt + AHL/wt (ES) | -3.8 | -2.1 |
| II 0838 | Succinoglycan Biosynthesis Transport Protein, ExoT | wt + AHL/wt (ES) | -1.7 | -4.3 |
| II 1116 | LuxR Family Transcriptional Regulator, VjbR | wt + AHL/wt (SP) | -2.9 | - |
| I 1758 | LuxR Family Transcriptional Regulator, BlxR | wt + AHL/wt (SP) | 27.5 | - |
| I 0155 | Putative Allantoin Permease | wt + AHL/wt (SP) | -1.7 | -1.4 |
| II 0025 | Attachment Mediating Protein VirB1 | wt + AHL/wt (SP) | -2.5 | -2.2 |
| II 0753 | ABC-Type Sorbitol/Mannitol Transport Inner Membrane Protein | ΔvjbR/ΔvjbR + AHL (ES) | 1.5 | 2.5 |
| I 1758 | LuxR Family Transcriptional Regulator, BabR | ΔvjbR/ΔvjbR+AHL (SP) | 99.5 | - |
A (-) indicates genes excluded for technical reasons or had a fold change of less than 1.5. qRT-PCR values were calculated by the ΔΔCt method normalized to 16s rRNA and are relative to the wildtype, averaged from 3 independently isolated samples, performed in triplicate in a minimum of three assays. ES, Exponential growth phase; SP, Stationary growth phase.