Table 2.
Transcripts associated with gene regulation significantly altered between 16M and 16MΔvjbR, with and without the treatment of C12-HSL to cells.
| BME Loci | Gene Function | Exponential Growth Phase Change (fold) | Stationary Growth Phase Change (fold) | STM | ||||
|---|---|---|---|---|---|---|---|---|
| ΔvjbR/wt | wt+AHL/wt | ΔvjbR/ΔvjbR+AHL | ΔvjbR/wt | wt+AHL/wt | ΔvjbR/ΔvjbR+AHL | |||
| I 0019 | LacI Family | -2.9 | -1.8† | - | 1.9 | 1.5† | - | |
| I 0305 | DeoR Family | -1.7 | -1.7† | - | 1.9 | 1.5† | - | [31] |
| I 0447 | Leucine-Responsive Regulatory Protein | 1.6 | - | - | -2.4 | -1.8 | - | |
| I 0781 | DNA-Directed RNA Polymerase A Subunit | 2.4† | 2.8 | - | - | - | - | [34] |
| I 1383 | AraC Family | -2.4 | -1.5† | - | - | -1.7† | - | |
| I 1607 | LuxR Family DNA Binding Domain | 1.8† | 3.0 | - | -1.5† | - | - | |
| I 1631 | TetR Family | -1.9 | -2.1 | - | - | - | - | |
| I 1700 | Predicted Transcriptional Regulator | 2.0 | 2.9 | - | - | - | - | |
| II 0051 | LuxR Family DNA Binding Domain | -1.9 | -2.8 | - | - | - | - | |
| II 0800 | AraC Family | 1.7 | 2.2† | - | - | - | - | |
| II 0854 | CRP Family Transcriptional Regulator | - | 1.6† | - | -1.5 | -1.7 | - | |
| II 0985 | LacI Family | -2.5 | -2.7† | - | -2.4 | - | - | |
| II 1022 | IclR Family | -1.5† | -1.8 | - | -1.9 | -2.1 | - | |
| II 1098 | AraC Family | -1.8 | -2.8 | - | 1.9 | 1.5 | - | |
| I 0446 | MarR Family | 1.9† | 2.9 | 2.9† | - | - | - | |
| I 0518 | Cold Shock Protein, CspA | 1.6 | - | -2.0† | 1.7 | - | - | |
| I 0720 | Sugar Fermentation Stimulation Protein | - | -2.0 | 1.7† | -1.7† | - | 1.5† | |
| I 0899 | Phage-Related DNA Binding Protein | -1.8 | -1.5† | -1.9† | 1.6 | - | -2.4† | |
| I 1098 | AsnC Family | -1.7 | -2.0 | -1.6† | -1.6 | - | - | |
| I 1291 | AraC Family | - | -1.9 | -1.7† | 1.7 | - | - | |
| I 1641 | TetR Family | - | - | -2.7† | -1.7 | -1.8 | - | |
| I 1885 | LysR Family | - | -1.8† | -2.3† | -1.6 | - | - | |
| II 0127 | IclR Family | - | 1.6† | - | -1.8 | - | 1.6† | |
| II 0219 | IclR Family | -3.2 | -5.8 | -3.8† | -1.5† | - | - | |
| II 0657 | Transcription Elongation Factor | 2.4† | 3.1 | - | - | - | 2.4† | |
| II 0810 | ArsR Family | - | 2.0 | - | 1.8 | 1.6† | -2.3† | |
A (-) indicates genes excluded for technical reasons or had a fold change of less than 1.5; † genes that did not pass the statistical significance test but showed an average alteration of at least 1.5-fold. Fold change values are the averaged log2 ratio of normalized signal values from two independent statistical analyses. Abbreviations as follows: STM, Signature Tagged Mutagenesis.