Table 4.
Genetic loci transcripts significantly altered between 16M and 16MΔvjbR, with or without the treatment of C12-HSL that may contribute to virulence.
| BME Loci | Gene Function | Exponential Growth Phase Change (fold) | Stationary Growth Phase Change (fold) | STM | ||||
|---|---|---|---|---|---|---|---|---|
| ΔvjbR/wt | wt + AHL/wt | ΔvjbR/ΔvjbR+ AHL | ΔvjbR/wt | wt + AHL/wt | ΔvjbR/ΔvjbR+ AHL | |||
| Cell Membrane | ||||||||
| I 1873 | Autotransporter Adhesin | -2.2 | - | - | - | - | - | |
| II 1069 | Adhesin, AidA | -1.5† | - | - | -1.5 | - | - | |
| I 0402 | 31 KDa OMP Precursor | - | 1.5† | - | -1.7 | -1.7† | - | |
| I 0330 | OpgC Protein | - | -2.0 | -1.9† | - | - | - | |
| I 0671 | Integral Membrane Protein, Hemolysin | - | -2.7 | -2.2† | - | - | - | [28] |
| II 1070 | Adhesin AidA-I | 1.7 | - | - | - | - | -1.9† | |
| I 1304 | Porin, F Precursor | - | - | -3.6† | -3.5 | -2.0 | -2.6† | |
| I 1305 | Porin | - | -2.3 | -1.8† | -1.5† | - | - | |
| Cell Motility | ||||||||
| II 0151 | Flagellar M-Ring Protein, FliF | -2.2 | -2.1 | - | 2.1† | - | - | [22,34] |
| II 0161 | Flagellar Hook-Associated Protein 3 | -1.8† | -2.7 | - | - | - | - | |
| II 0165 | Flagellar Biosynthesis Protein | -1.9† | -2.8 | - | - | - | - | |
| I 1692 | Flagellar Protein, FlgJ | - | - | -2.3† | -1.8 | -2.1 | -3.4† | |
| II 0160 | Flagellar Hook-Associated Protein, FlgK | -1.6† | -2.0 | -1.7† | - | - | - | |
| II 0162 | FlaF Protein | -2.1 | -2.0† | - | - | - | -1.6† | |
| II 0167 | Flagellar Biosynthesis Protein, FlhA | -1.6† | -2.3 | -1.8† | -1.5† | -1.9† | -5.5† | |
| II 1109 | Chemotaxis Protein, MotA | -1.6† | 2.0† | -3.6† | -1.7 | -1.5† | - | |
| Protease and Lipoprotein | ||||||||
| I 0611 | HflC Protein, Stomatin, Prohibitin, Flotillin, HflK-C Domains | -1.6 | - | - | - | -1.7† | - | |
| I 1079 | Lipoprotein NlpD | - | -1.5† | -1.6† | -1.6† | -1.9 | - | |
| I 1799 | Lipoprotein Signal Peptidase | 2.2 | 2.1† | - | - | -1.6† | - | |
| II 0831 | Hypothetical Protein, Aminopeptidase-Like Domain | -1.6† | -2.0 | - | -2.3 | - | 3.1† | |
| I 0213 | Metalloendopeptidase | -1.7† | -2.7† | -1.6† | 2.1 | - | - | |
| I 0282 | Zinc Metalloprotease | -1.8 | -1.7 | - | - | - | 3.4† | |
| II 0149 | Extracellular Serine Protease | -3.2 | -1.8 | 2.9† | - | -1.7 | - | |
| Secretion System | ||||||||
| I 0390 | VceA | -1.4† | -1.3† | - | - | -1.2† | - | |
| I 0948 | VceC | 1.1† | 1.4† | - | 1.6† | 1.3† | - | |
| I 1094 | Exopolysaccharide Production Negative Regulator Precursor, Tetratricopeptide Repeat | - | - | - | 2.1 | 1.5† | - | |
| I 1141 | Predicted Exported Protein | -1.6 | -1.7 | - | - | - | - | |
| I 1531 | Tetratricopeptide Repeat Family Protein | -2.1 | -2.4 | - | -1.7 | - | - | [34] |
| I 1077 | Hypothetical Exported Protein, YajC | -1.5 | -2.1 | - | -1.8† | -1.5† | 1.8† | |
| II 0025 | Attachment Mediating Protein VirB1 | -2.2 | -1.9 | - | -2.6 | -2.2 | - | [29,31,36] |
| II 0026 | Attachment Mediating Protein VirB2 | - | -2.1 | - | -4.3 | -3.6 | -1.3† | [29,31,36] |
| II 0027 | Channel Protein VirB3 | - | - | - | -3.9 | -3.2 | - | [29-31,36] |
| II 0029 | Attachment Mediating Protein VirB5 | -2.0 | - | 1.6† | -5.7 | -4.5 | -1.2† | [29-32] |
| II 0030 | Channel Protein VirB6 | - | - | -1.7† | -2.8 | -2.3 | - | [29-31,36] |
| II 0032 | Channel Protein VirB8 | -1.6† | - | 1.1† | -3.3 | -2.6 | - | [29,31,32,36] |
| II 0033 | Channel Protein VirB9 | - | - | - | -1.8 | -1.9 | - | [29,31,36] |
| II 0034 | Channel Protein VirB10 | - | -1.5 | - | -2.0 | -1.9 | - | [29,31,36] |
| II 0036 | OMP, OprF, VirB12 | - | - | - | -1.7 | -1.7 | - | [29,36] |
| II 0466 | Tetratricopeptide Repeat Family Protein | - | 2.3 | 2.2† | -1.5† | - | - | |
| Signal Transduction | ||||||||
| II 0011 | Transcriptional Regulatory Protein, HydG | -1.5† | -2.0 | - | - | - | - | [31] |
| II 1014 | Two Component Response Regulator | - | 1.7† | - | 1.6 | -1.5† | - | |
| I 0370 | Sensory Transduction Histidine Kinase | -1.7 | -2.1 | -2.2† | -1.6† | - | 2.1† | |
| I 0372 | Two-Component Response Regulator | 1.6† | - | -1.5† | 1.5† | 1.8 | - | |
| I 2034 | Sensor Protein, ChvG | - | -1.7 | -2.4† | -2.0 | -1.6 | - | |
| Stress Response | ||||||||
| I 0887 | Peptidyl-Prolyl Cis-Trans Isomerase | - | -1.7 | - | 1.7 | 1.6 | - | |
| I 1619 | Hsp33-Like Chaperonin | - | - | - | 1.8 | 1.6† | - | |
| II 0245 | Universal Stress Protein Family, UspA | -1.8 | -1.7 | -2.0† | -2.5 | -2.5 | - | |
A (-) indicates genes excluded for technical reasons or had a fold change of less than 1.5; † genes that did not pass the statistical significance test but showed an average alteration of at least 1.5-fold. Fold change values are the averaged log2 ratio of normalized signal values from two independent statistical analyses. Abbreviations are as follows: STM, Signature Tagged Mutagenesis; OMP, Outer Membrane Protein.