Table 4.
Primers used for amplification and sequencing of PRRSV (Lena) and PRRSV (US5)
| Target | Sequence | Location* | Direction | Target | Sequence | Location* | Direction |
|---|---|---|---|---|---|---|---|
| Lena | US5 | ||||||
| ORF2a | 5'-gtsacaccktatgattacg-3' | 11471-11489 | Forward | ||||
| 5'-tcatrccctattytgcacca-3' | 12642-12623 | Reverse | |||||
| ORF4 | 5'-cggcccaittccatccigag-3' | 12756-12775 | Forward | ORF4 | 5'-cggcccaittccatccigag-3' | 12756-12775 | Forward |
| 5'-cattcagctcgcataicgtcaag-3' | 13653-13631 | Reverse | 5'-cattcagctcgcataicgtcaag-3' | 13653-13631 | Reverse | ||
| ORF5 | 5'-tgcticatttcitgacacc-3' | 13418-13436 | Forward | ORF5 | 5'-tgcticatttcitgacacc-3' | 13418-13436 | Forward |
| 5'-accttaagigcitatatc-3' | 14190-14173 | Reverse | 5'-accttaagigcitatatc-3' | 14190-14173 | Reverse | ||
| ORF6 | 5'-atgggaggcctagacgatttt-3' | 14087-14107 | Forward | ORF6 | 5'-taccaacttcattgtggac-3' | 13922-13940 | Forward |
| 5'-ccggccatacttgacgaggt-3' | 14605-14586 | Reverse | 5'-acccagcaactggcacag-3' | 14690-14673 | Reverse | ||
| ORF7 | 5'-tggcccctgcccaicacg-3' | 14412-14429 | Forward | ORF7 | 5'-tggcccctgcccaicacg-3' | 14412-14429 | Forward |
| 5'-tcgccctaattgaataggtga-3' | 15050-15030 | Reverse | 5'-tcgccctaattgaataggtga-3' | 15050-15030 | Reverse |
*Locations are derived from Lelystad virus