Figure 6. D257A mice show decreased protein expression of both nuclear-encoded and mitochondrial-encoded ETC subunits.

(A) The content of selected nuclear-encoded (NDUFA9 and NDUFS3 subunits of Cx I, 29 kDa and 48 kDa subunits of Cx III), and mitochondrial-encoded subunits (COX I subunit of Cx IV) in skeletal muscle extracts of 11-mo old WT and D257A mice (n = 11 per group) were evaluated by Western Blotting. Representative blots are depicted. (B) Statistical analysis of ETC subunits protein expression. Arbitrary units represent densitometry values normalized to porin. Mean values ±SEM are shown. *p<0.05. Cx: complex.