Table 4.

Allele richness (R_s), expected heterozygosity (H_e) and F_ST estimates at flanking and neutral microsatellites of P. falciparum dhfr double and triple mutants

		dhfr genotypes
Microsatellites		Double mutant (N = 52)		Triple mutant (N = 99)		all samples (N = 151)
		Rs	He	Rs	He	Rs	He	FST	P
Loci flanking dhfr gene	Dhfr 0.3	8	0.34	7	0.68	9	0.51	0.38	<0.0001
	Dhfr 4.4	8	0.48	6	0.45	7	0.46	-0.01	NS
	Dhfr 5.3	6	0.35	6	0.37	7	0.36	0.57	<0.0001
	All loci	7	0.39	6	0.50	8	0.44	0.37	<0.0001
Loci flanking dhps gene	Dhps 0.8	12	0.67	10	0.53	13	0.60	0.01	NS
	Dhps 4.3	8	0.70	7	0.67	8	0.69	0.01	NS
	Dhps 7.7	14	0.92	15	0.90	17	0.91	-0.01	NS
	All loci	11	0.76	11	0.70	13	0.73	0.00	NS
Neutral loci	TAA81	8	0.83	11	0.84	10	0.83	0.02	NS
	TA109	10	0.76	11	0.72	11	0.74	0.01	NS
	TA87	11	0.89	12	0.85	12	0.87	0.01	NS
	PfPK2	10	0.85	13	0.88	13	0.87	-0.01	NS
	All loci	10	0.83	12	0.82	12	0.83	0.01	NS

N: Number of isolates genotyped. All loci: mean over loci R_s and H_e and global F_ST over loci as calculated by FSTAT. P: P-values of permutation tests to assess significance of F_ST values. NS: non-significant (P > 0.05). In bold: significant after Bonferroni corrections for multiple tests.