Table 5.
Allele richness (Rs), expected heterozygosity (He) and FST estimates at flanking and neutral microsatellites of P. falciparum dhps genotypes
| dhps genotypes | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Microsatellites | Wild type (N = 26) | Single mutant (N = 154) | Double mutant (N = 15) | all samples (N = 195) | |||||||
| Rs | He | Rs | He | Rs | He | Rs | He | FST | P | ||
| Loci flanking dhps gene | Dhps 0.8 | 8 | 0.91 | 4 | 0.47 | 5 | 0.79 | 6 | 0.71 | 0.25 | <0.0001 |
| Dhps 4.3 | 6 | 0.85 | 4 | 0.53 | 3 | 0.26 | 4 | 0.55 | 0.30 | <0.0001 | |
| Dhps 7.7 | 8 | 0.90 | 10 | 0.92 | 3 | 0.28 | 9 | 0.71 | 0.12 | 0.02 | |
| Over all loci | 7 | 0.82 | 6 | 0.63 | 4 | 0.45 | 6 | 0.66 | 0.22 | <0.0001 | |
| Loci flanking dhfr gene | Dhfr 0.3 | 5 | 0.68 | 6 | 0.72 | 5 | 0.76 | 6 | 0.72 | 0.02 | NS |
| Dhfr 4.4 | 4 | 0.49 | 5 | 0.44 | 2 | 0.13 | 5 | 0.36 | <-0.01 | NS | |
| Dhfr 5.3 | 5 | 0.66 | 5 | 0.63 | 3 | 0.53 | 5 | 0.61 | -0.01 | NS | |
| Over all loci | 5 | 0.61 | 5 | 0.60 | 3 | 0.47 | 5 | 0.56 | 0.01 | NS | |
| Neutral loci | TAA81 | 7 | 0.84 | 7 | 0.83 | 6 | 0.83 | 7 | 0.83 | 0.01 | NS |
| TA109 | 6 | 0.74 | 8 | 0.77 | 4 | 0.50 | 8 | 0.67 | 0.01 | NS | |
| TA87 | 9 | 0.87 | 9 | 0.87 | 7 | 0.87 | 9 | 0.87 | <0.01 | NS | |
| PfPK2 | 7 | 0.85 | 10 | 0.88 | 6 | 0.86 | 10 | 0.86 | 0.01 | NS | |
| Over all loci | 7 | 0.83 | 9 | 0.84 | 6 | 0.77 | 9 | 0.81 | 0.01 | NS | |
N: Number of isolates genotyped. All loci: mean over loci Rs and He and global FST over loci as calculated by FSTAT. P: P-values of permutation tests to assess significance of FST values. NS: non-significant (P > 0.05). In bold: significant after Bonferroni corrections for multiple tests.