Fig. 4.

Lensless multi-angle characterization of RBCs located within two-layered micro-channels. (a)–(e) Lensfree holograms are captured with five different illumination angles. The magenta dashed rectangles in (a)–(e) are the regions corresponding to the field-of-view shown in (g); and the yellow rectangles define the regions corresponding to the field of view of the images in Fig. 6. (f) The cross-sectional structure of the 2-layered sample is shown. (g) The 2D distribution of the RBCs located in both vertical channels is calculated with their height coded by the colormap. (h) shows the histogram of the cell heights over the entire field of view, which exhibits a double peaked behavior, as expected, resolving the 2 vertical micro-channels. In (g) and (h), the relative height is arbitrarily assumed to be 0 µm at the surface of the sensor.