Table 1. In situ Hybridization of DHHC and Thioesterase mRNA.

Gene	Locus	Template	Embryo3	Larval Brain4	Adult1
Thioesterases
Ppt1	8A2	cDNA:GM14257	N	N	Mag
CG4851/Ppt2	32E2	cDNA:GH02317	N	N	H, Cr
CG18815/Apt1	68D1	cDNA:GH04560	Mat, Neu, End, Mes	Y	Br, H, Tg, Cr, Mg
DHHC Transferases
CG1407	46D5-45D7	cDNA:LD36375	Mat	N	Br, H, Tg, Mg, Mag
CG4483	67A4	cDNA:AT26975	N	N	Tes2
CG4676	49F11	Genomic PCR	N	N	Br, Tes
CG4956	96F2	Genomic PCR	n.d.	n.d.	Tes2
CG5196	87B9	cDNA:GH28414	N	N	-
CG5620	69A2-69A4	cDNA:RE02357	Mat, Mes, End	Y	Mg, Mag, Tes
CG5880	97E2	cDNA:LD25420	N	N	Ov
CG6017	72C1	cDNA:LD10758	Mat, Neu, Mes	Y	Ov
CG6618	33C4	cDNA:GH15747	N	N	Br, H, Tg
CG6627	32B1-B2	cDNA:LD14687	Mat, Neu, End, SG	Y	Br, H, Ov
CG8314	52D9	cDNA:GH13672	N	N	Mg
CG10344	58E1	cDNA:AT16443	N	N	Tes, Ov
CG13029	73D3	cDNA:AT31918	N	N	Tes2
CG17075	21B6	Genomic PCR	N	N	Tes2
CG17195	96F2	Genomic PCR	n.d.	n.d.	Tes2
CG17196	96F2	Genomic PCR	N	N	Tes2
CG17197	96F2	Genomic PCR	N	N	Tes2
CG17198	96F2	Genomic PCR	n.d.	n.d.	Tes2
CG17257	23C5	cDNA:LD11027	Mat, Neu, End	Y	Br, Tg
CG17287	54A2	Genomic PCR	N	N	Tes2
CG18810	38D2	cDNA:AT08088	N	N	Tes2
CG34449	8D7-D8	RT-PCR	N	N	Ov

¹Adult Expression showing 2 fold or greater enrichment over whole fly was obtained from FlyAtlas (University of Glasgow) Affymetrix Chip Data (flyatlas.org). Abbreviations: Br, Brain; Cr, Crop; H, Head; TG, Thoracicoabdominal ganglion; Mg, Midgut; T, Tubule; H, Hindgut; Ov, Ovary; Tes, Testis; Mag, Male accessory gland

²Adult Expression was only detected in Testes according to FlyAtlas (University of Glasgow) Affymetrix Chip Data (www.flyatlas.org).

³Tissue enrichment during embryonic development. Abbreviations: End, Endoderm; Mat, Maternal; Mes, Mesoderm; Neu, Neural; SG, Salivary Gland; N: no observable expression, n.d.: not determined

⁴Enrichment in the larval brain as determined by in situ hybridization. N: no observable expression, Y; observable enrichment in brain.