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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Amino Acids. 2010 Mar 22;41(1):7–27. doi: 10.1007/s00726-010-0552-0

Fig. 7.

Fig. 7

De novo synthesis of stress responsive protein cytosolic HSP70i following TFEC exposure (100, 200 or 300 μM; 4h) to TAMH cells in cell culture. A. Quantitative real-time PCR determination of hsp70i mRNA with gene-specific primers. Data are represented as the fluorescence yield from successive PCR reaction cycles with the fold-induction indicated to the right (maximum of ≈ 400×). Data are the mean (± s.d.) from 3 independent experiments (n = 3-6 replicate plates per experiment). B. New synthesis of HSP70i protein also in TAMH cell cultures in vitro which paralleled hsp70i mRNA induction. Migration of Mr standards are shown to the left. Original and some additional (unpublished) data are from the study of Ho et al. (2006).