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. 2010 Apr 29;46(7):573–576. doi: 10.1007/s11626-010-9317-z

Figure 1.

Figure 1.

Attachment and proliferation of hES and iPS cells on type I collagen. The attachment of hES/iPS cells to ECM components was measured by the procedures followed by Fassler and Meyer (1995). Briefly, a 96-well microplate (Corning Costar, Corning, NY) was coated with each adhesion molecule at 37°C for 3 h. hES/iPS cells were seeded at confluent density (3 × 106 cells cm−2) on type I collagen (Nitta Gelatin, Inc.) coated plates in hESF9 (Cell Science & Technology Institute, Inc.). After 3 d, the attached cells were fixed and stained for 30 min with 0.4% crystal violet (Sigma) in methanol. After the plate was washed and dried, a solution (1% acetic acid and 30% ethanol in water) was added to the wells to dissolve the crystal violet. The absorbance of 595 nm, which indicated the concentration of the dissolved crystal violet, was measured with a microplate reader (model 550; Bio-Rad, Hercules, CA). Each graph shows the percentage of the attached cells on type I collagen in hESF9 relative to the attached cells on fibronectin (Sigma) as 100% as all the cell lines attached to fibronectin in hESF9. Bar = SE (n = 3).