Fig. 4.

Influence of MTX released from bone cement on the cell cycle of mesenchymal stem cells (a) with representative DNA analysis and cell cycle distribution in control (Ctrl) and methotrexate treated cells (MTX) with G2 blockage (b). Analysis was performed on the tenth day of incubation. Abbreviations: Ctrl control incubation without any pellet, Cem incubation with pellet without MTX, MTX01 incubation with pellet containing 1 mg of liquid MTX per gram of polymer powder, MTX10 incubation with pellet containing 1 mg of liquid MTX per gram of polymer powder