Table 1.
Morphometric data on mature resting cysts of Maryna umbrellata. CV – coefficient of variation in %, i – number of cysts investigated, LM – light microscopy, M – median, Max – maximum, Min – minimum, n – number of individual structures investigated, SD – standard deviation, SE – standard error of mean, SEM – scanning electron microscopy, TEM – transmission electron microscopy.
| Characteristicsa | Method | Unit | Mean | M | SD | SE | CV | Min | Max | n | i |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Trophic specimens, width (OsO4-fixed) | LM | μm | 105.1 | 100.0 | 19.2 | 3.3 | 18.3 | 75.0 | 140.0 | 34 | 34 |
| Trophic specimens, hight (OsO4-fixed) | LM | μm | 95.8 | 90.0 | 13.8 | 2.4 | 14.4 | 75.0 | 125.0 | 34 | 34 |
| Cysts, diameter in vivo (from environmental specimen) | LM | μm | 109.3 | 110.0 | 10.7 | 2.8 | 9.8 | 90.0 | 128.0 | 15 | 15 |
| Cysts, diameter in vivo (from cultivated specimens) | LM | μm | 102.5 | 100.0 | 8.8 | 1.9 | 8.6 | 90.0 | 120.0 | 21 | 21 |
| Cysts, diameter after Epon embedding (cultiv. specimens)a | LM | μm | 80.8 | 82.5 | 8.6 | 1.0 | 10.6 | 65.0 | 100.0 | 69 | 69 |
| Pericyst, total thickness |
TEM | μm | 5.7 | 5.5 | 1.0 | 0.3 | 17.1 | 4.4 | 7.7 | 9 | 9 |
| Basal layer, thickness |
TEM | μm | 1.7 | 1.2 | 0.8 | 0.3 | 48.7 | 0.9 | 3.4 | 9 | 9 |
| Basal layer, diameter of tubules | TEM | nm | 20.0 | 20.0 | 5.1 | 0.7 | 25.4 | 11.0 | 36.0 | 57 | 9 |
| Ectocyst, thickness |
TEM | nm | 140.0 | 122.0 | 65.3 | 21.8 | 46.7 | 71.0 | 286.0 | 9 | 9 |
| Mesocyst, thickness |
TEM | μm | 6.5 | 6.0 | 1.7 | 0.6 | 26.2 | 4.2 | 9.4 | 9 | 9 |
| Mesocyst, number of dark layers | TEM | – | 12.4 | 13.0 | 1.9 | 0.7 | 15.6 | 8.0 | 15.0 | 9 | 9 |
| Bright zone between meso- and endocyst, thickness | TEM | nm | 272.0 | 167.0 | 184.9 | 82.7 | 68.0 | 106.0 | 500.0 | 5 | 5 |
| Endocyst, thickness | TEM | nm | 512.8 | 490.0 | 237.1 | 79.0 | 46.3 | 222.0 | 900.0 | 9 | 9 |
| Cyst wall, total thickness in vivo (cultiv. spec.)b | LM | μm | 8.3 | 8.0 | 1.4 | 0.5 | 16.4 | 6.0 | 11.0 | 23 | 23 |
| Cyst wall, total thickness without glass layer | TEM | μm | 9.3 | 8.7 | 3.0 | 1.0 | 32.0 | 5.3 | 14.3 | 9 | 9 |
| Cyst wall, total thickness with glass layer | TEM | μm | 13.3 | 12.8 | 3.2 | 1.1 | 24.1 | 8.7 | 18.6 | 9 | 9 |
| Spongy globules, length |
TEM | μm | 4.3 | 4.4 | 1.1 | 0.2 | 24.5 | 2.5 | 6.3 | 45 | 9 |
| Spongy globules, width |
TEM | μm | 3.8 | 3.7 | 0.9 | 0.1 | 24.5 | 2.1 | 5.8 | 45 | 9 |
| Spongy globules, number of bright areas | TEM | - | 29.0 | 60.5 | 10.5 | 1.6 | 36.4 | 12.0 | 53.0 | 46 | 9 |
| Spongy globules, max. length of a bright area | TEM | nm | 640.7 | 649.5 | 186.8 | 27.5 | 29.2 | 300.0 | 1333.0 | 46 | 9 |
| Lipid droplets, length |
TEM | nm | 1161.1 | 1111.0 | 379.0 | 48.9 | 32.7 | 500.0 | 2000.0 | 60 | 7 |
| Lipid droplets, width |
TEM | nm | 1049.0 | 1039.0 | 325.0 | 42.0 | 31.0 | 500.0 | 1733.0 | 60 | 7 |
| Silicon granules, lengthc |
SEM | nm | 813.9 | 812.0 | 313.0 | 28.0 | 38.5 | 333 | 1667 | 129 | 9 |
| Silicon granules, widthc |
SEM | nm | 688.2 | 689.0 | 249.0 | 23.0 | 36.2 | 235 | 1481 | 129 | 9 |
a
From three cultures embedded over a period of six month.
b
With glass layer, which amounts for about half of wall thickness.
c
From Foissner et al. (2009).