Table 2.

Cytochemical investigation of resting cysts of Maryna umbrellata. − no reaction, + slight reaction, ++ distinct reaction, +++ strong reaction, PAS – Periodic Acid-Schiff reaction, TEMS – thin sections as used for transmission electron microscopy, UL – uranyl acetate and lead citrate-stained thin sections.

	Carbohydrates and mucosubstances						Proteins		Lipids	Glassa
	UL-stained TEMS	Bismuth-stained TEMS	Chitin	Iodine	PAS	Alcian blue	Acrolein	Protease digestion of TEMS	Sudan black	Hydrofluoric acid
Glass granules	++	++	−	+	−	+++	+	−	−	+++
Basal layer	++	++	−	−	++b	−	−	−	−	−
Ectocyst	+++	+++	−	?	?	?	?	−	−	−
Mesocyst	++	+++	−	−	−	−	++	+++	−	−
Endocyst	++	++	−	−	++		+	+	−	−
Spongy globules	++	+++	−	++	++	−	++	+++	−	−
Lipid droplets	++	+++	−	−	−	−	+ ?	++	+++	−
Cytoplasm	+	++	−	++	++	++	+	++	−	−

^aFor details, see Foissner et al. (2009).

^bClearly recognizable only in semithin sections (Fig. 25).