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(Scheme 10) shows the ligation product of the pentamer (RE-PNA)5 molecule after the complete DARinv reaction with the diene compound dimethyl-1,2,4,5-tetrazine-3,6-dicarboxylate derivatized with the fluorescent marker dansyl chloride [5-(dimethylamino)naphthalene-1-sulfonyl chloride] connected by an ethylene diamine linker. The synthesis procedure is documented in the footnote.6
