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. 2010 Jul 2;6(4):361–370. doi: 10.7150/ijbs.6.361

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 1 — Regulation of FBPase degradation by TCO89. All strains were glucose starved for indicated time (1day or 2 days). In the experiments, cell cultures were equally divided and two treatments were given as indicated. Same amount of total protein was loaded in the gel for every sample. A. Deletion of TCO89 in Vid-dependent FBPase degradation pathway. IB, Immuno-blotting; WT, w303; Δtco89 and Δbit61, deletion mutants. B. Deletion of TCO89 in proteasome-dependent FBPase degradation. C. The importation of FBPase into Vid vesicle in Δtco89 mutant. Pellet fraction (P) containing Vid vesicles while supernatant fraction (S) containing soluble proteins. D. GST pull-down assay showed the physical association of TCO89 with FBPase. IP, immuno-precipitation. E. Sucrose gradient analysis showed co-localization of TCO89, FBPase and Vid24 during FBPase degradation.

Fig 1 — Regulation of FBPase degradation by TCO89. All strains were glucose starved for indicated time (1day or 2 days). In the experiments, cell cultures were equally divided and two treatments were given as indicated. Same amount of total protein was loaded in the gel for every sample. A. Deletion of TCO89 in Vid-dependent FBPase degradation pathway. IB, Immuno-blotting; WT, w303; Δtco89 and Δbit61, deletion mutants. B. Deletion of TCO89 in proteasome-dependent FBPase degradation. C. The importation of FBPase into Vid vesicle in Δtco89 mutant. Pellet fraction (P) containing Vid vesicles while supernatant fraction (S) containing soluble proteins. D. GST pull-down assay showed the physical association of TCO89 with FBPase. IP, immuno-precipitation. E. Sucrose gradient analysis showed co-localization of TCO89, FBPase and Vid24 during FBPase degradation.