Fig 2.

The involvement of TORC1 in FBPase degradation. All strains were glucose starved for two days. In the experiments, the 2ml cell cultures were equally divided and different treatments were given as indicated. Same amount of total protein was loaded in the gel for every sample. A. FBPase degradation was rapamycin sensitive. Two-day starved cells (-) and starved cells supplied with glucose for 2hrs (+) were treated with ethanol or rapamycin (20nM) during FBPase degradation. B. Tor1 overexpression blocked FBPase degradation. WT: w303; Tor1 overexpression: ectopic expression of Tor1-V5; Δtor1, Δbit61, Δavo2, and Δavo3: deletion mutants for components of Tor Complex I and Tor complex II. C. Rapamycin did not relieve the impairment of FBPase degradation by Tor1 overexpression.