Table 1.
yeast strains in this study
| Strain | Genotype |
|---|---|
| W303 | MATa leu2,3-112 ade2 his3-Δ200 trp1-1 ura3-52 |
| HLY650 | MATαhisΔ1 leu2Δ0 lys2Δ0 ura3Δ0 |
| BY4742 | MATαhis3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 (Euroscarf, Germany) |
| Δtco89 | MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0 tco89::kanmx4 (Euroscarf, Germany) |
| Δarp8 | MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0 arp8::kanmx4 (Euroscarf, Germany) |
| Δbit61 | MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0 bit61::kanmx4 (Euroscarf, Germany) |
| Δavo2 | MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0 avo2::kanmx4 (Euroscarf, Germany) |
| Δavo3 | MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0 avo3::kanmx4 (Euroscarf, Germany) |
| Δtor1 | MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0 tor1::kanmx4 (Euroscarf, Germany) |
| GST-TCO89 | Matα his3-Δ200,leu2-3, 112, ura3-52, pep4::URA, GST-TCO89::LEU 23 |
| GST-ARP8 | Matα his3-Δ200,leu2-3,112, ura3-52,pep4::URA, GST-ARP8::LEU 23 |
| Tor1-V5-HIS | Matα his3-Δ200 ura3-52 leu2, 3-112 trp1-1, Tor1 -V5-HIS URA( this study) |
| FBPase -V5-HIS | Mata his3-Δ200 ura3-52 leu2,3-112 trp1-1, FBPase -V5-HIS URA( this study) |
| PLY298 | MATa ade2-1 trp1-1 can1-100 leu2-3 112 his3-11, 15 ura3 GAL+3HA-TOR1: His3MX6 22 |
| PLY122 | MATa ade2-1 trp1-1 can1-100 leu2-3 112 his3-11, 15 ura3 GAL+ 3HA-TOR2: His3MX6 22 |
| PLY307 | MATa ade2-1 trp1-1 can1-100 leu2-3 112 his3-11, 15 ura3 GAL+ LST8-HA:TRP1 22 |
| PLY306 | MATa ade2-1 trp1-1 can1-100 leu2-3 112 his3-11, 15 ura3 GAL+ KOG1-HA: TRP1 22 |
| PLY336 | MATa ade2-1 trp1-1 can1-100 leu2-3 112 his3-11, 15 ura3 GAL+TCO89-MYC:TRP1 22 |
| HLY2100 | MATa ade2-1 trp1-1 can1-100 leu2-3 112 his3-11, 15 ura3 GAL+3HA- TOR1: His3MX6, FBPase -V5-HIS LEU( this study) |
| HLY2101 | MATa ade2-1 trp1-1 can1-100 leu2-3 112 his3-11, 15 ura3 GAL+ 3HA-TOR2:His3MX6, FBPase -V5-HIS LEU( this study) |
| HLY2102 | MATa ade2-1 trp1-1 can1-100 leu2-3 112 his3-11, 15 ura3 GAL+ LST8-HA: TRP1 FBPase -V5-HIS LEU( this study) |
| HLY2103 | MATa ade2-1 trp1-1 can1-100 leu2-3 112 his3-11, 15 ura3 GAL+ KOG1-HA: TRP1 FBPase -V5-HIS LEU( this study) |
| HLY2104 | MATa ade2-1 trp1-1 can1-100 leu2-3 112 his3-11, 15 ura3 GAL+ 3HA-TOR1:His3MX6, P1S fbpase -V5-HIS LEU( this study) |
| HLY2105 | MATa ade2-1 trp1-1 can1-100 leu2-3 112 his3-11, 15 ura3 GAL+ 3HA-TOR2:His3MX6, P1S fbpase -V5-HIS LEU( this study) |
| HLY2106 | MATa ade2-1 trp1-1 can1-100 leu2-3 112 his3-11, 15 ura3 GAL+ LST8-HA: TRP1, P1S fbpase -V5-HIS LEU( this study) |
| HLY2107 | MATa ade2-1 trp1-1 can1-100 leu2-3 112 his3-11, 15 ura3 GAL+ KOG1-HA: TRP1, P1S fbpase -V5-HIS LEU( this study) |