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. Author manuscript; available in PMC: 2010 Jul 8.
Published in final edited form as: Biochemistry. 1991 Apr 30;30(17):4257–4261. doi: 10.1021/bi00231a022

FIGURE 4.

FIGURE 4

19F NMR spectra of folded and unfolded 5F-Trplabeled receptor and of aqueous 5F-Trp. All samples contained 100 mM KCl, 10 mM Tris, pH 7.1 with HCl, 1 mM D-glucose, and 10% D2O; samples B–E also contained 200–500 μM 5F-Trp-labeled receptor. Individual samples contained (A) 10 mM 5F-Trp, free amino acid, (B) 3 M guanidine HCl, (C) heat treatment at 67.5 °C for 1 h prior to and during spectral accumulation, (D) 20 mM EDTA, and (E) 0.5 mM CaCl2. Other sample and spectral parameters at 470 MHz were as in Figure 1.