Fig. 4.

A typical two-dimensional gel image used in analysis. SYPRO orange-stained two-dimensional gel image of plasma samples taken from standard chow diet control mouse (8 weeks) is shown. Approximate pI range and molecular weight are labeled along the top and left-hand side borders of the gel, respectively. The gel image was captured using a laser-scanner (Pharos FX plus, Bio-Rad) with the excitation–emission setting at 473–520 nm. Each rectangle indicates an area of protein isoform distribution described in Figs. 5, 6, 7, and 8. Spot detection and quantification of each protein spot was performed using the PDQuest 7.0.1 program (Bio-Rad)