Table 1.
List of protein identification parameters
| Protein name (isoform number) | Experimental pI | Ion score | Coverage (%) | Peptide sequence |
|---|---|---|---|---|
| RBP 4 (1)a | 5.3 | 65 | 4 | K.YWGVASFLQR.G |
| 70 | 10 | K.KDPEGLFLQDNIIAEFSVDEK.G | ||
| RBP 4 (2) | 5.5 | 67 | 4 | K.YWGVASFLQR.G |
| 72 | 10 | K.KDPEGLFLQDNIIAEFSVDEK.G | ||
| RBP 4 (3) | 5.7 | 66 | 4 | R.FSGLWYAIAK.K |
| 74 | 10 | K.YWGVASFLQR.G | ||
| RBP 4 (4) | 7.0 | 71 | 4 | K.YWGVASFLQR.G |
| 70 | 10 | K.KDPEGLFLQDNIIAEFSVDEK.G | ||
| Transthyretin (1)b | 5.3 | 66 | 10 | K.TAESGELHGLTTDEK.F |
| 89 | 15 | K.TLGISPFHEFADVVFTANDSGHR.H | ||
| Transthyretin (2) | 5.8 | 119 | 15 | K.TLGISPFHEFADVVFTANDSGHR.H |
| 65 | 16 | R.HYTIAALLSPYSYSTTAVVSNPQN | ||
| Transthyretin (3) | 6.0 | 127 | 15 | K.TLGISPFHEFADVVFTANDSGHR.H |
| 70 | 16 | R.HYTIAALLSPYSYSTTAVVSNPQN | ||
| Transthyretin (4) | 6.3 | 70 | 2 | K.DVFLGTFLYEYSR.R |
| 105 | 16 | K.TLGISPFHEFADVVFTANDSGHR.H | ||
| Transthyretin (5) | 6.4 | 157 | 16 | K.TLGISPFHEFADVVFTANDSGHR.H |
| 116 | 15 | R.HYTIAALLSPYSYSTTAVVSNPQN | ||
| Transthyretin (6) | 6.6 | 130 | 16 | K.TLGISPFHEFADVVFTANDSGHR.H |
| 97 | 15 | R.HYTIAALLSPYSYSTTAVVSNPQN | ||
| Apolipoprotein A1 (1)c | 5.5 | 74 | 3 | K.WKEDVELYR.Q |
| 67 | 4 | K.VAPLGAELQESAR.Q | ||
| Apolipoprotein A1 (2) | 5.6 | 74 | 3 | K.WKEDVELYR.Q |
| 67 | 4 | K.VAPLGAELQESAR.Q | ||
| Apolipoprotein A1 (3) | 5.7 | 74 | 3 | K.WKEDVELYR.Q |
| 88 | 4 | K.VAPLGAELQESAR.Q | ||
| Apolipoprotein A1 (4) | 5.8 | 74 | 3 | K.WKEDVELYR.Q |
| 76 | 4 | K.VAPLGAELQESAR.Q | ||
| Apolipoprotein A1 (5) | 5.9 | 74 | 3 | K.WKEDVELYR.Q |
| 76 | 4 | K.VAPLGAELQESAR.Q | ||
| Apolipoprotein A1 (6) | 6.0 | 74 | 3 | K.WKEDVELYR.Q |
| 67 | 4 | K.VAPLGAELQESAR.Q | ||
| Kininogen (1)d | 5.5 | 73 | 2 | K.TDGSPTFYSFK.Y |
| 90 | 4 | K.EVLGHSIAQLNAENDHPFYYK.I | ||
| Kininogen (2) | 5.6 | 70 | 2 | K.TDGSPTFYSFK.Y |
| 90 | 4 | K.EVLGHSIAQLNAENDHPFYYK.I |
Two ion scores from MS/MS analysis considered for identification of each protein spot are tabulated above
a
Protein name: Plasma retinol-binding protein precursor—mouse; Accession code: BAB25881; Nominal mass: 20386; Calculated pI value: 4.98
b
Protein name: Transthyretin precursor—mouse; Accession code: VBMS; Nominal mass: 15766; Calculated pI value: 5.77
c
Protein name: Apolipoprotein A-I precursor—mouse; Accession code: JC1237; Nominal mass: 30526; Calculated pI value: 5.51
d
Protein name: High molecular weight kininogen variant—mouse; Accession code: Q6S9I1_MOUSE; Nominal mass: 53172; Calculated pI value: 4.88