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. Author manuscript; available in PMC: 2010 Jul 8.
Published in final edited form as: Proteomics. 2009 Mar;9(5):1220–1229. doi: 10.1002/pmic.200800473
All targets = Target hits only (above threshold)
False positives (FP) = Decoy hits
True positives (TP) = All targets above threshold - FP
False discovery rate (FDR) = FP / FP + TP

Example for 1000 identifications, 20 decoy identifications.

All targets = 980 (1000-20)

FP = 20

TP = 960 (980-20)

FDR = 2.04% (20 / 980)