All targets | = Target hits only (above threshold) |
False positives (FP) | = Decoy hits |
True positives (TP) | = All targets above threshold - FP |
False discovery rate (FDR) | = FP / FP + TP |
Example for 1000 identifications, 20 decoy identifications.
All targets = 980 (1000-20)
FP = 20
TP = 960 (980-20)
FDR = 2.04% (20 / 980)