Figure 7.

Treatment of Wild-Type Cells with GC Inhibitors LY and ODQ Leads to Partial Release of NIA1, NRT2.1, AMT1.1, and AMT1.2 Expression in Media Containing Nitrate and Ammonium: Effect of the NO Scavenger cPTIO.

The effect of LY83,583 (6-anilino-5,8-quinolinedione) and ODQ (1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one) on the expression of the indicated genes was determined by real-time PCR. Wild-type 704 was grown in minimum medium containing 8 mM NH₄Cl. After 3 d of growth, cells were induced during 1 h in minimum medium containing 4 mM KNO₃ + 1mM NH₄Cl supplied LY (A) or ODQ (B) at the concentrations indicated. Alternatively (C), expression of these genes was determined in cells induced during 1 h in minimum medium containing 100 μM KNO₃ in the absence or presence of NONOate 100 μM (used as controls). The effect of 100 μM cPTIO was analyzed either after preincubating the cells 15 min with cPTIO previously to NONOate addition (protocol 1) or when added simultaneously with DEA NONOate at time 0 (protocol 2). The error bars represent the sd of three biological replicates.