Figure 10.

Opposite Regulation of NRT1.8 and NRT1.5 Expression under Stress Conditions.

(A) Four-week-old plants were exposed to 200 μM Cd²⁺ for 6 h or were kept under control conditions (CK). NRT1.5 expression was determined in stems (S), rosette leaves (L), and roots (R) by RNA gel blots. 18S rRNA was used as a loading control.

(B) Quantitative RT-PCR of NRT1.5 expression in plants treated with 20 μM (L) or 200 μM (H) Cd²⁺ for the indicated times. n = 3, and values are mean ± sd. Fold change compared with control (CK) is shown.

(C) Expression data of NRT1.8/At4g21680 and NRT1.5/At1g32450 were obtained from Genevestigator (Zimmermann et al., 2004). Examples representing the following stresses were selected: osmotic (mannitol), cold (low temperature), MJ (methyl jasmonate), P.syringae (Pseudomonas syringae), salt (NaCl), and heavy metal (AgNO₃). Fold change compared with control (CK) is shown. Detailed information and more data are available from Genevestigator (https://www.genevestigator.com/gv/index.jsp).