Figure 6.

NRT1.8 Expression under Cd²⁺ Stress.

(A) and (B) NRT1.8 expression was determined in roots (A) and shoots (B) by RNA gel blotting after plants were exposed to 0, 40, and 200 μM Cd²⁺ treatment for the indicated times. 18S rRNA was used as a loading control.

(C) Quantitative RT-PCR analysis of NRT1.8 expression in plants exposed to 20 μM (L) and 200 μM (H) Cd²⁺ for the indicated times. The y axis shows NRT1.8 RNA levels normalized to that of Actin2. n = 3, and values are mean ± sd. CK, control condition.