Figure 1.

Phenotypes of the rol Mutant.

(A) A wild-type spikelet. le, lemma; pa, palea.

(B) A rol spikelet with the rod-like lemma (arrowhead).

(C) A rol spikelet without a lemma. Arrowhead and arrow indicate a rudimentary organ (arrested lemma) and curled anthers, respectively.

(D) A rol spikelet with an elongated awn (arrow).

(E) and (F) rol spikelets rescued by introducing an 8.6-kb genomic fragment of the SHL2 gene. In the rescued transgenic plant, the development of the lemma and stamens is similar to that in the wild type.

(G) and (H) Adaxial (G) and abaxial (H) epidermal surface of the lemma in the wild type.

(I) Epidermal surface of the rod-like lemma in rol.

(J) A cross section of the lemma in the wild type. ab, abaxial side; ad, adaxial side.

(K) A cross section of the rod-like lemma in rol.

(L) and (M) In situ localization of ETT3 (L) and PHB3 (M) in the rod-like lemma of rol.

Bars = 1 mm in (A) to (F), 100 μm (G) to (I), and 50 μm (J) to (M).