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. Author manuscript; available in PMC: 2011 Jul 6.
Published in final edited form as: Biochemistry. 2010 Jul 6;49(26):5570–5581. doi: 10.1021/bi100656j

FIGURE 5.

FIGURE 5

Knocking down μ-calpain in FA-A cells by siRNA restores levels of αIISp to those found in normal cells. (A) FA-A cells were transiently transfected with 200, 240 or 300 pM μ-calpain siRNA or nontarget (Nt) siRNA. 48 hours after transfection, levels of μ-calpain and αIISp in the FA-A and normal cells were examined by western blot analysis. Immunoblots were probed with anti-μ-calpain and anti-αIISp. Tubulin (*) was used as a loading control. (B) Immunoblots were scanned and levels of μ-calpain and αIISp quantitated. Vertical lines represent ± s.e.m. for 5–8 experiments.