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. Author manuscript; available in PMC: 2011 Jul 6.
Published in final edited form as: Biochemistry. 2010 Jul 6;49(26):5570–5581. doi: 10.1021/bi100656j

FIGURE 7.

FIGURE 7

siRNA knockdown of μ-calpain in FA-A cells leads to formation of αIISp and XPF nuclear foci after damage with MMC. (A) FA-A cells were transfected with non-target siRNA (−) or 300 pM μ-calpain siRNA (+). Normal cells were transfected with Nt siRNA (−) or 300 pM μ-calpain siRNA (+). 24 hr after transfection, cells were either undamaged or treated with MMC (400 nM). Formation of αIISp and XPF nuclear foci was examined 16 hr after MMC treatment using indirect immunoflourescence and staining with anti-αIISp or anti-XPF. (B) The percentage of the nuclei showing multiple αIISp and XPF nuclear foci (> 10 foci) in normal and FA-A cells after transfection with non-target (Nt) siRNA or μ-calpain siRNA and MMC treatment was quantitated. 300 cells were counted in each group for each experiment. Vertical lines represent ± s.e.m. for 5 experiments.