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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Mol Cell Biochem. 2009 May 15;331(1-2):31–40. doi: 10.1007/s11010-009-0142-1

Figure 5.

Figure 5

The effects of calcium chelators on fatty acid-mediated induction of ER stress, cell death and cytochrome release in H4IIE liver cells and primary hepatocytes. H4IIE liver cells or primary rat hepatocytes were incubated for 16 hours in control media (LG), or control media supplemented with oleate (O, 250 µM), linoleate (L, 250 µM), palmitate (P, 250 µM) or stearate (S, 250 µM) in the absence (no additions) or presence of BAPTA-AM (20 µM) or EGTA (1 mM). (A) Real time PCR analysis of GRP78 mRNA where LG (no additions) was set to 1. (B) ELISA-based cell death. (C) ELISA-based assay for cytochrome c protein in post-mitochondrial supernatant fractions. Data are reported as the mean ± SD for triplicate samples from 4–6 independent experiments. *, significantly (p<0.05) different from LG-. +, significantly (p<0.05) different from No Additions of the same treatment group.