Skip to main content
. 2010 May 6;153(3):956–969. doi: 10.1104/pp.110.155259

Figure 4.

Figure 4.

Identification of ISA activities in 20-DAP endosperm extracts. A, Zymogram activity gels following MonoQ separation. Equal volumes of each fraction were analyzed. B, Correspondence of ISA proteins with ISA enzyme activities. Extracts were concentrated by AEC on Q Sepharose and separated by native-PAGE. ISA activities were visualized in zymograms, and ISA proteins were identified by immunoblot analysis. Zymograms are from a single gel, and immunoblots are from a second gel run simultaneously in the same apparatus. Numbers below each lane indicate micrograms of protein loaded. C, Independent repeat analysis of ISA activities in wild-type and isa2-339 endosperm. Details are as in B. D, Correspondence of ISA proteins with ISA enzyme activities in su1-P endosperm. Details are as in B.