Table I. Growth of E. coli BCAT knockouts complemented with SlBCATs.
Slope is measured as change in OD600 per hour. All strains were grown on minimal medium lacking amino acids.
Variable | Wild Type | Knockout | SlBCAT3 | SlBCAT4 | SlBCAT1 | SlBCAT2 |
Slope | 0.159 | 0.0004 | 0.083 | 0.062 | 0.029 | 0.017 |