Figure 2.

Increased sensitivity of the ptlpd1-1 mutant to As(V). A to C, Five-day-old wild-type (WT) and ptlpd1-1 seedlings germinated on solid medium (see legend to Fig. 1) were transferred to medium containing the indicated As(V) concentrations and allowed to grow for 12 d in a vertical orientation. Mean values ± se (n = 4 plates; two to four seedlings from each plate were pooled for each replicate) are presented for fresh weight (A), shoot anthocyanin concentration (B), and shoot-to-root fresh weight (FW) ratio (C). D, ptlpd1-1 and wild-type seeds were germinated on solid medium containing the indicated As(V) concentrations and scored for the emergence of green cotyledons after 5 d. Values are means ± se (n = 4 plates of 50–100 seeds).