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Subcellular localization of PIN-GFP proteins in the Arabidopsis root hair cell. A and B, Root hair specifically expressed PIN-GFP (ProE7:PIN-GFP; PIN5 = ProE7:PIN5-GFP1) fusion proteins were visualized by confocal microscopy. Root hair cells were visualized by only GFP signal (A) or by both GFP and FM4-64 dye signals (B). GFP and FM4-64 signals at the plasma membrane were merged for PIN3 and PIN8, whereas they were not for PIN5 (B). Bars = 10 μm for A and 5 μm for B.
