Figure 2.

Functional complementation of Saccharomyces cerevisiae mutant YBR029c lacking CDP-DAG synthase activity by expression of Arabidopsis CDS cDNAs under control of the PGAL promoter. Yeast suspensions of wild-type (WT) and mutant strain containing one of the given Arabidopsis CDS cDNAs were stepwise diluted 10 times and spotted on 2% Gal (A) and 2% Glc (B) and incubated at 28°C for 48 h. cDNAs are termed according to the proteins they encode (see Fig. 1). Unlike wild-type cells, mutant cells were only able to grow when expression of the Arabidopsis gene constructs was induced by Gal.