Expression pattern of AtDOF4.7. A, RT-PCR analysis of AtDOF4.7 expression in root, stem, leaf, flower, and siliques from 4-week-old Arabidopsis plants and 1-week-old whole seedlings. ACTIN (At3g18780) was used as the internal control gene. B, Expression of PromoterAtDOF4.7::GUS. Reporter gene expression in plants harboring the PromoterAtDOF4.7::GUS fusion is as follows: GUS activity in transgenic inflorescences (a); GUS expression restricted to the base of the floral organ in the siliques at position 6 (b); GUS expression in leaf trichomes (c); GUS expression in flowers at position 2 (d); GUS expression in the style of position 2 flowers (e); GUS expression at the base of pedicels (f); and a closer view of siliques at position 6 showing GUS expression in floral organ AZ (g). Bars = 1.5 mm. C, Time course expression of PromoterAtDOF4.7::GUS. The time course of GUS expression from different developmental positions of flowers and siliques of 5-week-old Arabidopsis is shown. The flowers were counted along the inflorescence from position 1, assuming that the flower at position 1 had visible white petals at the top of the inflorescence.