Figure 2.

Molecular identification of AtDOF4.7 overexpression plants. A, Southern hybridization of DNA from two AtDOF4.7 transgenic lines (S107 and S109) and the wild type (WT). Genomic DNA samples were digested with restriction enzymes HindIII and EcoRI and were then hybridized with a Hygromycin Phosphotransferase probe. B, Identification of AtDOF4.7 overexpression in 35S::AtDOF4.7 transgenic lines. The relative expression level of AtDOF4.7 was analyzed by quantitative real-time PCR from 4-week-old wild-type and transgenic plants. ACTIN was used as the internal control gene.