Figure 7.
GmRIN4a and GmRIN4b mediate basal immunity in soybean. A, Bacterial counts in rpg1-b (cv Essex) plants silenced for GmRIN4a (S4a) or GmRIN4b (S4b) and those inoculated with the empty silencing vector (V). Plants were infiltrated with virulent (vir) or avirulent (avrB) strains of P. syringae pv glycinea. Log10 values of colony-forming units (cfu) per unit of leaf area from infected leaves at 0 d post inoculation (dpi; white bars) or 4 dpi (black bars) are presented. Error bars indicate sd (n = 5). Student's t test was used to determine statistical significance. Asterisks denote significant differences from V plants inoculated with vir bacteria as follows: ** P < 0.001, *** P < 0.0001. Significant difference between avrB-inoculated V and S4a plants is denoted by a, where P < 0.05. Significant difference between vir and avrB-inoculated S4b plants is denoted by b, where P < 0.0001. B, Percentage survival of V (white squares), S4a (gray circles), or S4b (black triangles) plants at 1, 5, 7, 8, 9, 12, and 15 d post inoculation with P. sojae. Error bars indicate sd (n = 3). Asterisks denote significant differences from V, where P < 0.001, as determined using Student's t test. Results are representative of three independent experiments.