Table II. Kinetic parameters of wild-type and mutant reCCS.
The values shown are means ± se for three determinations. ND, Not determined.
| Enzyme | Vmax | Km | Relative Activity |
| nmol mg−1 h−1 | μm | % | |
| Wild type | 82 ± 6 | 3.7 ± 0.05 | 100 |
| D127A | 62 ± 3 | 4.2 ± 0.03 | 75.6 ± 4 |
| E128A | 3.6 ± 0.04 | 4.1 ± 0.04 | 4.4 ± 0.03 |
| D259A | 25.0 ± 6 | 3.8 ± 6 | 30.5 ± 2 |
| E295A | 0 | – | – |
| E295K | 0 | – | – |
| E295R | 0 | – | – |
| E296A | 1.2 ± 0.01 | ND | 1.5 ± 0.03 |
| E296K | 0.1 ± 0.01 | ND | 0.1 ± 0.01 |
| E296R | 0.1 ± 0.01 | ND | 0.1 ± 0.01 |
| E332A | 8.3 ± 0.4 | 3.5 ± 0.04 | 10.1 ± 0.5 |
| H360A | 0 | – | – |
| H360K | 25.0 ± 2 | 3.6 ± 0.05 | 30.5 ± 2 |
| H360R | 22.0 ± 2 | 3.8 ± 0.03 | 26.8 ± 1 |