Fig. 2.

Pterostilbene inhibits the expression of β-catenin, cyclin D1, COX-2, iNOS and phospho-p65 protein in the colon tumors. The colon tumor sections were processed and incubated with the respective primary antibodies as described in the Materials and Methods. β-Catenin and cyclin D1 staining was high in the cytosol and also present in the nucleus to a lower extent. iNOS and COX-2 showed cytoplasmic staining, whereas nuclear staining was predominant with phospho-p65. n = 3 per group for each analysis. A representative section is shown. Image magnification, ×400.