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. Author manuscript; available in PMC: 2011 Jul 6.
Published in final edited form as: Biochemistry. 2010 Jul 6;49(26):5524–5532. doi: 10.1021/bi1005199

Figure 3.

Figure 3

Characterization of immunopurified chondrocyte-derived perlecan. Immunopurified chondrocyte-derived perlecan was electrophoresed through 3 – 8 % SDS-PAGE gels, electroblotted onto a PVDF membrane and probed with an anti-perlecan antibody (Domain IV: MAb A7L6) (A). Immunopurified chondrocyte-derived perlecan was coated onto 96 well plates (B) and probed with antibodies against perlecan (Domain IV: MAb A7L6), heparan sulfate (HS, MAb 10E4), chondroitin sulfate (CS, MAb CS56) or keratan sulfate (KS, MAb 5D4). Data is presented as mean ± standard deviation (n=3).