Table 1.
Primary antibodies used in this study.
| Primary Antibody | Antigen | Immunogen | Source | Dilution |
|---|---|---|---|---|
| SMI32 | Neurofilament heavy non-phosphorylated | hippocampal protein extract | Covance (Emeryville, CA), mouse monoclonal, #SMI-32R, lot #9 | 1:400 |
| GFAP | Glial fibrillary acidic protein | GFAP purified from normal human brain | Sigma (St Louis, MO), rabbit polyclonal, #G9269 | 1:400 |
| MBP | Myelin basic Protein | Human MBP isolated from normal human brain. Rat monoclonal raised against full length bovine MBP. | Abcam (Cambridge, MA), rabbit polyclonal (#ab12355-50, lot #212221) or rat monoclonal (#ab7349-2, lot #226183) | 1:400 |
| NG2 | NG2 chondroitin sulfate proteoglycan | Immunoaffinity purified NG2 chondroitin sulfate proteoglycans from rat | Chemicon (Billerica, MA), rabbit polyclonal, #ab5320, lot #LV1361596 | 1:400 |
| APC | Adenomatus polyposis coli | Recombinant protein consisting of amino acids 1–226 of APC | Calbiochem (San Diego, CA), mouse monoclonal, #OP80, lot #D35078 | 1:200 |
| Collagen IV | Collagen Type IV | Human and bovine placental collagen IV | Chemicon (Billerica, MA),goat polyclonal,#ab769, lot#24020212 | 1:400 |