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. 2010 Jun 30;5(6):e11400. doi: 10.1371/journal.pone.0011400

Figure 1. DCs viability and activation depend of the MOI of nrMVA.

Figure 1

DCs were infected with nrMVA at different MOIs for 6 h. After washing and further incubation for 16 h, changes in the expression of surface molecules on non-infected and nrMVA-infected DCs were measured by flow cytometry. DC viability was assessed by FACS using Annexin-V (apoptotic cells) and 7-AAD (necrotic cells) after infection with nrMVA at different MOIs. The MOI of 0.05, 0.5 and 5 were arbitrarily considered as representative of low, intermediate and high MOI, respectively. Data are representative of 3 independent experiments.